PREPARATION OF INOCULUM Inoculate 50 ml of BBL Trypticase Soy Broth with a loop of. Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI). Maintain it by monthly transfer on Trypticase Soy Agar (BBL) slants.BBL LIM Broth, 5ml Tube, 100/pk (Perishable Product Must be Refrigerated. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. BD BBL Trypticase Soy Agar (TSA II) with Defibrinated Sheep Blood.The 150 × 15 mm-style plates of TrypticaseSoy Agar are convenient for use with Taxofactor strips in the isolation and differentiation of Haemophilusspecies. Unless otherwise stated, sterilize media by autoclaving at 121C for 15 min. It is not the medium of choice for anaerobes. TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines. Tryptic (Trypticase) Soy Agar (TSA) is used for the isolation and cultivation of nonfastidious and fastidious microorganisms.Using sterile 1.0 mL pipettes, inoculate tubes with 0.75 mL of the dilutions. This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products. From 24- to 48-h Trypticase Soy Broth cultures or Enriched Thioglycollate Medium cultures for the Bacteroides and Clostridium strains, prepare a dilution containing 100 or less CFU/mL.TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. BD Tryptic Soy Broth (Soybean-Casein Digest Medium) is a general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for. Trypticase Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.
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